Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
Identifieur interne : 000093 ( Main/Exploration ); précédent : 000092; suivant : 000094Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
Auteurs : Jesse J. Waggoner ; Victoria Stittleburg ; Renee Pond ; Youssef Saklawi ; Malaya K. Sahoo ; Ahmed Babiker ; Laila Hussaini ; Colleen S. Kraft ; Benjamin A. Pinsky ; Evan J. Anderson ; Nadine RouphaelSource :
- Emerging infectious diseases [ 1080-6059 ] ; 2020.
Abstract
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
DOI: 10.3201/eid2607.201285
PubMed: 32294051
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.</div>
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<name sortKey="Hussaini, Laila" sort="Hussaini, Laila" uniqKey="Hussaini L" first="Laila" last="Hussaini">Laila Hussaini</name>
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<name sortKey="Pinsky, Benjamin A" sort="Pinsky, Benjamin A" uniqKey="Pinsky B" first="Benjamin A" last="Pinsky">Benjamin A. Pinsky</name>
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<name sortKey="Waggoner, Jesse J" sort="Waggoner, Jesse J" uniqKey="Waggoner J" first="Jesse J" last="Waggoner">Jesse J. Waggoner</name>
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